Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil
نویسندگان
چکیده
In the Brazilian Amazon region, malaria caused by Plasmodium malariae is considered to be a zoonosis because of cross-transfer of the parasite between humans and Neotropical primates. To contribute information on this issue, we investigated occurrences of natural infection with Plasmodium sp. among Neotropical primates in the Maranhense Amazon (Amazon region of the state of Maranhão), in the northeastern region of Brazil. Blood samples were collected from 161 Neotropical primates of six species that were caught in an environmental reserve (Sítio Aguahy) and from captive primates (CETAS-Wildlife Screening Center, municipality of São Luís), in Maranhão. Plasmodium sp. was diagnosed based on light microscopy, PCR, qPCR and LAMP for amplification of the 18S rRNA gene. Serum samples were also assayed by means of indirect immunofluorescence for IgG antibodies against P. malariae/P. brasilianum, P. falciparum and P. berghei. Parasites were detected through light microscopy on five slides from captive primates (four Sapajus spp. and one Callithrix jacchus). In the molecular tests, 34.16% (55/161) and 29.81% (48/161) of the animals sampled were positive in the qPCR and PCR assays, respectively. In the PCR, 47/48 animals were positive for P. malariae/P. brasilianum; of these, eight were free-living primates and 39 from CETAS, São Luís. One sample showed a band in the genus-specific reaction, but not in the second PCR reaction. Anti-P. malariae/P. brasilianum IgG antibodies were detected in four serum samples from Sapajus spp. in captivity. In this study, circulation of P. malariae/P. brasilianum in Neotropical primates was confirmed, with low levels of parasitemia and low levels of antibodies. The importance of these animals as reservoirs of human malaria in the region studied is still unknown. This scenario has an impact on control and elimination of malaria in this region.
منابع مشابه
Natural Plasmodium infection in neotropical primates in the island of São Luís, state of Maranhão, Brazil Infecção natural de Plasmodium em primatas neotropicais da Ilha de São Luís, Estado do Maranhão, Brasil
The states that make up the Legal Amazon Region, which include the state of Maranhão, account for 99% of registered cases of human malaria in Brazil. It is also believed that transmission of malaria from nonhuman primates (NHP) to humans occurs in this region, because of current reports of seroepidemiological results from samples from humans and NHP coexisting in the same areas. This study aime...
متن کاملGreen Synthesis of Silver Nanoparticles Using Amazon Fruits
In this study, we report the green synthesis of silver nanoparticles (AgNPs) from extracts of native fruits from Amazonia, Brazil. AgNPs were characterized by UV/Vis and medium infrared (MIR) spectroscopy. Their antimicrobial activities were evaluated against the growth of bacteria and leavers, as well as the evaluation of minimum inhibitory concentration (MIC) and minimum bactericidal c...
متن کاملFirst record and description of a new species of Sycorax Curtis (Diptera: Psychodidae, Sycoracinae) from the Brazilian Amazon.
Sycorax longispinosa sp. nov. is described from Serra do Cachorro, Pará State, Brazil. This new species is the first record of the genus from the Brazilian Amazon. An identification key for males of species of Sycorax from the neotropical region is presented.
متن کاملSEROLOGICAL DETECTION OF HEPATITIS A VIRUS IN FREE-RANGING NEOTROPICAL PRIMATES (Sapajus spp., Alouatta caraya) FROM THE PARANÁ RIVER BASIN, BRAZIL
Nonhuman primates are considered as the natural hosts of Hepatitis A virus (HAV), as well as other pathogens, and can serve as natural sentinels to investigate epizootics and endemic diseases that are of public health importance. During this study, blood samples were collected from 112 Neotropical primates (NTPs) (Sapajus nigritus and S. cay, n = 75; Alouatta caraya, n = 37) trap-captured at th...
متن کاملSevere Plasmodium falciparum malaria Malária grave por Plasmodium falciparum
Malaria is the world’s leading parasitic disease and affects approximately 40% of the world’s population (approximately 2.4 billion people) in more than 100 countries.(1) The disease, that is caused for Plamodium spp., particularly affects populations in tropical and subtropical areas, especially in developing and underdeveloped countries. Brazil has the largest number of cases on the American ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 12 شماره
صفحات -
تاریخ انتشار 2017